Enhanced separation of DNA restriction fragments by capillary gel electrophoresis using field strength gradients.

Standards commonly employed to calibrate &e excludon chromrrto@raphy cdunn, usually do not t h e ” have welldefined &os. Carefully characterized spherkal solutes In the apporprlate rlze rHI(J. are therefore of condderabk Interest. The chromatographk khavlor of carboxylated starburst dendrhers-characterlzed by quaslelaatk lbht scatterlng and vkomotry-on a Super080 &e exdudon cdunnhasbnnexpbred. Cahoxylatedstart&a’std”m appear to behave as nonlnteractlng spheres durlng chromatography In the prwmce of an approprlate aqwow moblk phase. The dependence of the retention t h e on solute rlze seem8 to cdnclde wlth data collected on the same column for Flcoll, an uncharged, quad-apherkal pdysaccharlde. Chromatography of the dondrbnen yhkk a remarkable correlation of the chromatographk partltlon coeMccknt with the generation n m k , thk result k in part a comquence of the expornntlal relathehlp between the generatton number and the molecular volume of the dendrimenr.